Molecular mechanisms of Tanshinone IIA in Hepatocellular carcinoma therapy via WGCNA-based network pharmacology analysis
نویسندگان
چکیده
Hepatocellular carcinoma (HCC) is a worldwide malignant tumor that caused irreversible consequences. Tanshinone IIA has been shown to play notable role in HCC treatment. However, the potential targets and associating mechanism of against remain unknown. We first screened out 105 overlapping genes by integrating predicted from multiple databases differentially expressed Cancer Genome Atlas (TCGA) database. Then, we performed weighted gene co-expression network analysis (WGCNA) using RNA-seq profiles HCC-related clinical information. 23 related grade important module were imported for Gene Ontology (GO) enrichment, Kyoto Encyclopedia Genes Genomes (KEGG) protein-protein interaction (PPI) analysis. Comparing key WGCNA with high connectivity nodes PPI network, identified three hub genes, AURKB, KIF11, PLK1. For further verification, tested binding genes. The survival curve, receiver operating characteristic (ROC) mRNA expression, protein expression also used validate In study, revealed grade-specific modules, following KEGG pathway indicated probably plays therapeutical effect development HCC, especially cell cycle. Our result partially explained pharmacological HCC.
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OBJECTIVE To explore the possible mechanisms of Tanshinone IIA (TanIIA) on esophageal carcinoma cell lines. METHODS Two human esophageal carcinoma cell lines (EC-1 cells and ECa-109 cells) were treated with different concentrations of TanIIA. Cell proliferation was measured by CCK-8, colony-forming efficiency was calculated, cell cycle and apoptosis were measured, and changes in cell cycle- a...
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Hepatocarcinogenesis is a complex process that remains still partly understood. That might be explained by the multiplicity of etiologic factors, the genetic/epigenetic heterogeneity of tumors bulks and the ignorance of the liver cell types that give rise to tumorigenic cells that have stem cell-like properties. The DNA stress induced by hepatocyte turnover, inflammation and maybe early oncogen...
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ژورنال
عنوان ژورنال: Biocell
سال: 2022
ISSN: ['0327-9545', '1667-5746']
DOI: https://doi.org/10.32604/biocell.2022.018117